Genome Project Solutions
BAC Library Vectors Used
The BAC clone libraries SymBio distributes are cloned into one of these vectors:
pBACe3.6 map and information at CHORI, Sequence and features
pTARBAC2.1 map and information at CHORI , Sequence and features
pcc1BAC information from supplier (pdf)
Major pBACe3.6 and pTARBAC2.1 Vector Characteristics:
1. loxP site
2. sacBII gene allows for positive selection.
3. pUC plasmid region disrupts the sacBII gene and allows for high copy number.
4. 6 sites can be used: SacI, SacII, MluI, EcoRI, BamHI and AvaIII.
5. Chloramphenicol resistance (grow at 20ug/ml)
6. Ampicillin resistance (grow at 100ug/ml)
7. T7 & SP6 primer sites
8. Not I sites for cutting out the insert
References:
Frengen, E. et al. (1999) A Modular, Positive Selection Bacterial Artificial Chromosome Vector with Multiple Cloning Sites. Genomics 58: 250-253.
Shizuya H. et. al. (1992) Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector.Proc. Natl. Acad. Sci. USA 89:8794-8797
Pierce et al. (1992) A positive selection vector for cloning high molecular weight DNA by the bacteriophage P1 system: improved cloning efficacy.Proc. Natl. Acad. Sci. USA 89:2056-2060Back to BAC Clone Main Page
